Macro Pharma flow cytometry technology share

      Flow Cytometry (FCM) is a technique that uses flow cytometry to qualitatively and quantitatively analyze and sort a variety of fluorescently labeled single cells or other biological particles at a functional level. At present, it is mainly used in immune cell typing detection, phenotype detection, intracellular factor detection, cell killing, proliferation, apoptosis, cycle detection, antigen-specific cell detection, absolute cell count and cell sorting.

    Immune cell typing is the most common application of flow cytometry. Immunotyping is based on the specific binding of various fluorescent antibodies to the unique cell surface differentiation antigens of lymphocytes, and then the cells are analyzed by cell size, particle complexity and fluorescence intensity using flow cytometry.

    Most immune cells have specific CD markers, such as T lymphocyte markers (CD3, CD4, CD8), B lymphocyte markers (CD19, CD20), monocyte markers (CD14, CD11b), and NK cell markers (CD56, CD16); There are also Treg cells (FoxP3, CD127), Th1 cells (IFN-γ, TNF-α, IL-2), Th2 cells (IL-4), Th17 cells (IL-17A), proliferation markers (Ki67, CFSE), activation markers (CD69, CD25), Memory markers (CD45RO, CD27), chemokine receptor markers (CCR7, CCR5, CXCR4, CCR6), etc.

    The level of lymphocyte is an important indicator of cellular and humoral immune function of the body, and any disease or disease factor (sub-health) will be manifested by the change of lymphocyte level.

    When the amount and function of different lymphocyte subsets change abnormally, the body will produce a series of pathological changes and immune dysfunction, so that bacteria, viruses and other foreign pathogenic substances can easily invade the body to cause disease, or autoimmune diseases. Measuring the changes of human lymphocyte subsets is very important for early detection and control of these diseases, guiding clinical treatment and evaluating the immune status of the body.